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TMT based proteomics

3

Description

Isobaric tags labeling technique was firstly applied for the simultaneous identification and relative quantification of peptide pairs by Thompson et al. in 2003. Tandem Mass Tag (TMT) system was designed by Thermo Fisher Scientific for the identification and quantification of proteins in different kinds of samples. Each TMT tagging reagent is composed of an amine-reactive NHS-ester group, a spacer arm and an MS/MS reporter. And the isobaric tagging reagent within a set has the same nominal parent (precursor) mass. These reporter ions are in the low mass region of the MS/MS spectrum and are used to report expression changes in cell-based or tissue samples that may not be amenable to metabolic isotopic labeling strategies such as SILAC.

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Listing ID: 160619738e6105fa

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